Share icon Three circles with dashes Person icon Man with pen You Tube Logo Just "You tube" text Facebook logo Small letter f Search icon Magnifier Twitter logo Simplified small bird Email icon Envelope
Skip to main
bacteria background render

Genomic Characterization of Murine Monocytes Reveals C/EBPβ Transcription Factor Dependence of Ly6C− Cells

Monocytes are circulating, short-lived mononuclear phagocytes, which in mice and man comprise two main subpopulations

bacteria background render

3d render illustration of colorful bacteria

Authors:
Mildner, A; Schonheit, J; Giladi, A; David, E; Lara-Astiaso, D; Lorenzo-Vivas, E; Paul, F; Chappell-Maor, L; Priller, J; Leutz, A; Amit, I; Jung, S

Abstract:
Monocytes are circulating, short-lived mononuclear phagocytes, which in mice and man comprise two main subpopulations. Murine Ly6C+ monocytes display developmental plasticity and are recruited to complement tissue-resident macrophages and dendritic cells on demand. Murine vascular Ly6C− monocytes patrol the endothelium, act as scavengers, and support vessel wall repair. Here we characterized population and single cell transcriptomes, as well as enhancer and promoter landscapes of the murine monocyte compartment. Single cell RNA-seq and transplantation experiments confirmed homeostatic default differentiation of Ly6C+ into Ly6C− monocytes. The main two subsets were homogeneous, but linked by a more heterogeneous differentiation intermediate. We show that monocyte differentiation occurred through de novo enhancer establishment and activation of pre-established (poised) enhancers. Generation of Ly6C− monocytes involved induction of the transcription factor C/EBPβ and C/EBPβ-deficient mice lacked Ly6C− monocytes. Mechanistically, C/EBPβ bound the Nr4a1 promoter and controlled expression of this established monocyte survival factor.

Full Article

____________________________________________________________________________________________________________________________

Israel BDS – building dialogue through science – aims to promote the kind of international collaboration that can lead to true understanding between people. Israel BDS stands for the free and open exchange of ideas among scientists everywhere. By reporting on the benefits of Israeli-international scientific research and the web of connections that these scientists create around the world, Israel BDS takes a vibrant approach to highlighting the global necessity of continued international scientific collaboration.

___________________________________________________________________________________________________________________________